WebAug 16, 2024 · Background Acidithiobacillus caldus, a Gram-negative, chemolithotrophic sulfur-oxidizing bacterium, is widely applied in bioleaching. The absence of an ideal selection marker has become a major obstacle to achieve high efficiency of the gene transfer system for A. caldus. Plasmid pJRD215, widely used in Acidithiobacillus spp., … WebAug 16, 2024 · Conclusion: Chloramphenicol was proved to be an ideal selection marker for A. caldus. Novel plasmids carrying cat gene were constructed. The utilization of these vectors will undoubtedly facilitate efficient genetic manipulations and accelerate the research progress in A. caldus. MeSH terms Acidithiobacillus / drug effects
Chloramphenicol - Wikipedia
WebMay 13, 2024 · One colony each of DH1PEPA (pBRT1Nc), DH1 (pBRT1Nc) or WT05 (pBRT1Nc) were picked from chloramphenicol selective plates and reinoculated into 5 ml of LB or LB–aro mix without antibiotic selection and cultured for approximately 16 h … WebNov 16, 2013 · We routinely use the chloramphenicol InTag adaptor for IgG reformatting of ‘Fab-on-phage’ clones, although InTag adaptors with a different selection marker (e.g. zeocin) can also be used. We also use InTag positive selection cloning for further optimization of lead IgG antibody candidates using heavy or light chain shuffling … surrogate mother legal countries
Construction of novel pJRD215-derived plasmids using chloramphenicol …
WebChloramphenicol shows a potent but delayed parasiticidal effect, allowing the use of E. coli chloramphenicol acetyl transferase (CAT) not only as reporter enzyme but also as a … WebUsing Ampicillin as a Selection Marker for Plasmid DNA Isolation. There are several selectable markers that can be used to identify bacterial cells that contain a specific trait. … WebChloramphenicol is a potent inhibitor of the cytochrome P450 isoforms CYP2C19 and CYP3A4 in the liver. [33] Inhibition of CYP2C19 causes decreased metabolism and therefore increased levels of, for example, antidepressants, antiepileptics, proton-pump inhibitors, and anticoagulants if they are given concomitantly. surrogate mother program ukraine