WebSubcloning Efficiency™ DH5α™ Competent Cells are tested for transformation efficiency using the pUC19 control DNA supplied with the kit and using the protocol on page 2. Transformation efficiency should be greater than 1 x 106 transformants/µg pUC19 DNA. … WebStandard efficiency (propagation) Subcloning Efficiency™ DH5α™9 (1 x 106 cfu/µg) gDNA or library production One Shot™ OmniMAX 2 T1R (>20 kb) ElectroMAX ™ DH10BOne Shot (>20 kb) ElectroMAX ™ DH10B T1R (>20 kb) MegaX DH10B ™ T1R Electrocomp ™8 (>100 kb) High transformation efficiency (cloning) One Shot ™ …
DH5α Competent Cells for Subcloning - Thermo Fisher Scientific
WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5 … WebMar 16, 2024 · Economical for routine subcloning procedures or applications where starting DNA is not limiting. Brand: Invitrogen™ 18265017. Additional Details : Weight : … teach me hausa language
MAX Efficiency DH5 Competent Cells - Harvard University
WebDH5 is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5 increase insert stability and imp ... WebMar 16, 2024 · Economical for routine subcloning procedures or applications where starting DNA is not limiting. Brand: Invitrogen™ 18265017. Additional Details : Weight : 0.05000kg. Product Code. 11583117. 83.25 € / 2mL. Quantity. Add to basket. Includes: Subcloning Efficiency DH5α Competent Cells: 4 vials, 500μL each (total of 2mL), pUC19 DNA … Web5 Using DH5α-T1R as a Transient Host Introduction DH5α-T1R competent E. coli support replication of M13mp vectors, but it does not support plaque formation. Plating on a lawn of E. coli containing the F plasmid will allow plaque formation. Before Starting You will need the following reagents: • Log-phase E. coli containing the F plasmid (e.g. DH5α-FT™, … teach mandarin