Hoechst 33342 apoptosis
NettetUsing Hoechst 33342 staining, we observed increased numbers of apoptotic monocytes within 6 h of infection with M. avium subsp. paratuberculosis, and these numbers … NettetHoechst 33342, 10 mg/mL in Water: 40046: 561.93: 10 mL: 350/461 nm: Yellow solution: Hoechst 33342, trihydrochloride trihydrate: 40047: 615.98: 100 mg: 350/461 nm: …
Hoechst 33342 apoptosis
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Nettet您好！欢迎来到炼石商城 请登录 注册 我的订单; 我的炼石 NettetThe adenosine-induced apoptosis was determined by Hoechst 33342 staining and flow cytometry. The effect of adenosine on the intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) was assessed according to the DCFH-DA and JC-1 methods, respectively.
NettetProtocol Analyzing Cell Death by Nuclear Staining with Hoechst 33342 Lisa C. Crowley, Brooke J. Marfell, and Nigel J. Waterhouse Cold Spring Harb Protoc;2016; … Nettet10. okt. 2024 · Hoechst 33342 dye was used to assess morphological changes of apoptosis. Expression levels of Akt, FoxO3a, GSK-3β, and apoptosis- and cell cycle-associated proteins were detected by Western blotting. Tube formation assay was used to test the angiogenesis role of conditioned medium from MSCs in vitro. The cytokine …
Nettet18. feb. 2024 · For this experiment, the mechanism for which cell death ensues can be elucidated by the use of two different stains: Hoechst 33342 and propidium iodide. In … NettetApproximate fl uorescence excitation/emission maxima, when bound to DNA: ~350/461 nm Hoechst dye; ~535/617 nm propidium iodide Chromatin Condensation/Dead Cell …
Nettet1. sep. 1994 · Abstract A flow cytometric method to detect apoptotic cells is described. This method is based on the detection of differences in chromatin condensation with Hoechst 33342 as a probe and the detection of dead cells with propidium iodide as a probe for membrane damage.
Nettet15. des. 2024 · To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups. Hoechst 33342 trihydrochloride purchased from MCE. 飯塚 お寿司Nettet16. sep. 2024 · A staining dye solution was prepared by adding 1 µL of calcein AM (4 mM stock from Invitrogen), 1 µL Hoechst 33342, and 10 µL CellEvent ® Caspase-3/7 into 8 mL of staining buffer. The staining dye solution was dispensed at 1 µL per microwell in the microwell chips which was then combined with the micropillar chip containing the cells … 飯塚 お宮参り 写真Nettet31. okt. 2024 · First, we monitored the distribution of nuclear contents by staining human Jurkat T cells with the cell-permeable DNA binding dye Hoechst 33342 prior to induction of apoptosis. Next, cells... 飯塚 お持ち帰りNettetIn this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at long times in the absence of imaging at high dye concentrations, phototoxicity from repeated excitation of the dye in the imaging process is dominant. 飯塚 お寿司 テイクアウトNettetHoechst 33342, a vital DNA-specific fluo-rochrome, was added for determinations of DNA content and subsequent cell cycle analysis (Fig. 1). There is a decrease in the Hoechst 33342 fluorescence in the apop-totic population compared with the nonapoptotic popu-lation. The incorporation of the intracellular tandem GFP-FRET 飯塚 お笑いNettetHoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. It also has been used to detect DNA in solution between 250ng/ml to … 飯塚 お笑いタレントNettetThrough hematoxylin-eosin (HE) staining, Hoechst 33342 staining, transmission electron microscopy, and flow cytometry using FITC-Annexin V/PI double staining, 2,5-HD was … 飯塚 お祭り