WebHere we investigated phagocytic plasticity of THP-1 (acute monocytic human leukemia) cells at different stages (days 1, 3, and 7) of phorbol ester (PMA)-induced macrophage … WebJul 28, 2024 · In contrast, THP-1 cells were minimally responsive or unresponsive. Despite no NTP-dependent decrease in cell surface display of CD47 in either cell line, both cell …
miR-148a Affects Polarization of THP-1-Derived Macrophages and …
Web1 day ago · FcγR expression of THP-1-CD16A macrophages was 168 evaluated by flow cytometry using fluorescent antibodies to FcγRI (clone 10.1), FcγRIIA 169 (clone IV.3), FcγRIIA/B/C (clone AT10), and FcγRIIIA/B (clone 3G8). 170 In vitro macrophage phagocytosis of ITP serum-opsonized platelets 171 Human macrophage phagocytosis … WebMay 25, 2015 · CD47 was more highly expressed on THP-1 LSCs (P < 0.05) and was an independent predictor of survival and refractory disease in THP-1-engrafted mice. … holiday inn electric road roanoke va
Antibody:CD47 ratio regulates macrophage phagocytosis through …
WebThus, we next investigated whether the enhanced migration of THP-1 cells to conditioned medium is dependent on Ccr2. To this end, we disabled Ccr2 using an anti-Ccr2 antibody and performed a transwell migration assay. Blockade of Ccr2 with an anti-Ccr2 antibody appreciably diminished migration of THP-1 cells to conditioned medium (Figure 3f). WebMar 6, 2024 · For phagocytosis assays in which the interactions in cis on THP-1 cells was disrupted, we also added 1.5 µM anti-CD47 clone B6H12 antibody to the THP-1 cells prior to the addition of RBCs. The antibody was allowed to bind for 1 h prior to the assay and then … WebBut sometimes cells deattach during the polarization process (48h). Here is the protocol, which I am using: 1. Seeding THP-1 cells (1,3x10 6 / well) in 6-well plates (2ml RPMI … holiday inn elizabethtown pa