THP-1 (TIB-202 from ATCC) cells were cultured in RPMI1640 + Glutamax-I Medium (Gibco, 61870-010) with 10% FCS (Invitrogen, 50064), 1% sodium pyruvate (Gibco, 11360-070) and 1% penicillin/streptomycin (Gibco, 15140-122) at 37 °C and 5% CO2. Cells were passaged twice per week and kept at a cell … See more Design, construction, and basic performance of the genome-wide sgRNA library has been described in Michlits et al.21. To generate focused sgRNA pools for … See more Lentiviral vectors were produced using MISSION Lentiviral Packaging Mix (Sigma-Aldrich), PEI Max (Polysciences, Inc.) and Lenti X-293T cells (Clontech … See more THP-1 iCas9 cells were transduced with lentiviral particles using spinfection. To this end, THP-1 cells were transferred into 6-well plates, virus was added and … See more FACS-enriched or baseline cells were collected by centrifugation, washed with PBS and a maximum of 5E6 cells were resuspended in 350 µL DNA extraction … See more WebMar 6, 2024 · For phagocytosis assays in which the interactions in cis on THP-1 cells was disrupted, we also added 1.5 µM anti-CD47 clone B6H12 antibody to the THP-1 cells prior …
Selection of reliable reference genes during THP-1 monocyte ...
WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as … Web5 IRF1) or alternative activation markers (CCL17, ALOX15, CCL26, TGM2, CD200R and CD206).Similar conditions can be used for polarizing PMA-primed THP-1 cells with IL-10 … m and s matching pjs
THP-1 cells as a model for human monocytes - Bosshart - Annals …
WebJul 16, 2024 · In contrast, THP-1 macrophages did not phagocytize live, unopsonized and intra-erythrocytic mature stage V gametocytes . Gametocytes were previously detected in … WebFigure 1. (A) Experimental outline for comparative kinome profiling of undifferentiated (monocytic) and differentiated (macrophage-like) THP-1 cells. Cells were metabolically … Web2 days ago · An increase in exposure of β-(1,3)-glucan and chitin on the cell surface was also observed through flow cytometry. Interestingly, we observed a decrease in the … m and s marzipan fruits