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Thp-1 phagocytosis

THP-1 (TIB-202 from ATCC) cells were cultured in RPMI1640 + Glutamax-I Medium (Gibco, 61870-010) with 10% FCS (Invitrogen, 50064), 1% sodium pyruvate (Gibco, 11360-070) and 1% penicillin/streptomycin (Gibco, 15140-122) at 37 °C and 5% CO2. Cells were passaged twice per week and kept at a cell … See more Design, construction, and basic performance of the genome-wide sgRNA library has been described in Michlits et al.21. To generate focused sgRNA pools for … See more Lentiviral vectors were produced using MISSION Lentiviral Packaging Mix (Sigma-Aldrich), PEI Max (Polysciences, Inc.) and Lenti X-293T cells (Clontech … See more THP-1 iCas9 cells were transduced with lentiviral particles using spinfection. To this end, THP-1 cells were transferred into 6-well plates, virus was added and … See more FACS-enriched or baseline cells were collected by centrifugation, washed with PBS and a maximum of 5E6 cells were resuspended in 350 µL DNA extraction … See more WebMar 6, 2024 · For phagocytosis assays in which the interactions in cis on THP-1 cells was disrupted, we also added 1.5 µM anti-CD47 clone B6H12 antibody to the THP-1 cells prior …

Selection of reliable reference genes during THP-1 monocyte ...

WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as … Web5 IRF1) or alternative activation markers (CCL17, ALOX15, CCL26, TGM2, CD200R and CD206).Similar conditions can be used for polarizing PMA-primed THP-1 cells with IL-10 … m and s matching pjs https://boudrotrodgers.com

THP-1 cells as a model for human monocytes - Bosshart - Annals …

WebJul 16, 2024 · In contrast, THP-1 macrophages did not phagocytize live, unopsonized and intra-erythrocytic mature stage V gametocytes . Gametocytes were previously detected in … WebFigure 1. (A) Experimental outline for comparative kinome profiling of undifferentiated (monocytic) and differentiated (macrophage-like) THP-1 cells. Cells were metabolically … Web2 days ago · An increase in exposure of β-(1,3)-glucan and chitin on the cell surface was also observed through flow cytometry. Interestingly, we observed a decrease in the … m and s marzipan fruits

Human serum albumin-based probes for molecular targeting of …

Category:THP-1 and human peripheral blood mononuclear cell

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Thp-1 phagocytosis

Phagocyte Chemoattraction Is Induced through the Mcp-1–Ccr2 …

WebTHP-1 cells (ATCC®, TIB-202™, ATCC, Manassas, VA, USA), ... (10 μM) (Sigma-Aldrich) were used to block phagocytosis in THP-1 macrophages. Cells were preincubated with … WebJun 7, 2012 · The inhibitory role of CD47 on erythrocyte phagocytosis by THP-1 can be demonstrated by pretreating the erythrocytes with F(ab′) 2 fragment of the B6H12 …

Thp-1 phagocytosis

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WebIn the present study, we analyze the phagocytosis of wasteosomes and the mechanisms involved in this process. Accordingly, we purified wasteosomes from post-mortem … WebM2-polarized macrophages exhibit a higher phagocytic capacity compared with THP-1 cells and treatment with Terb reduces phagocytosis. The capacity of taking up FITC-dextran …

WebBoth phagocytosis and efferocytosis, two fundamental functions of macrophages, ... Loss of ME-1 in THP-1 cells was also associated with reduced efferocytosis. ME-1 is a target … WebJun 1, 2024 · Good day, I am having problems with culturing my THP-1 cells. I tried culturing them in several different volumes, I was recommended to seed 5,000,000 cells in 100mL …

WebMonociles and their macrophage and dendritic-cell progeny serve three main functions the the immune system. Those have phagocytosis, antigen showcase, and cytokine … WebBut sometimes cells deattach during the polarization process (48h). Here is the protocol, which I am using: 1. Seeding THP-1 cells (1,3x10 6 / well) in 6-well plates (2ml RPMI …

WebThe surface morphology of the cobalt chrome particles but not the quartz particles was changed after phagocytosis by THP-1 cells. This study suggests that the genotoxic and …

WebFeb 28, 2024 · The degree of autophagy and phagocytosis of THP-1 cells to aged erythrocytes are both downregulated when suppressing the NLRP3 expression on THP-1 … m and s maternity swimwearWebJan 8, 2024 · Phagocytosis assay. The differentiated THP-1 cells (2 × 10 5 cell/well) were treated with 10 μg/mL fluorescein isothiocyanate (FITC)-labelled MSNPs for 2 h at 37 °C. … m and s maternity nightwearWebThe results indicate that phagocytosis via FcγRIIa is unaffected by IL-4 treatment (88 ± 4 SRBC ingested by non treated THP-1 cells and 85 ± 3 SRBC ingested by IL-4-treated THP … korean actors 200 official - instagramWebIn the coculture system, THP-1 cells were seeded on six-well plates at a density of 1×10 6 cells per well and treated with 10 µM PMA for 48 h to form THP-1 macrophages (THP-M … m and s maternity leggingsWebtamm-horsfall glycoprotein enhances pmn phagocytosis by binding to cell surface-expressed lactoferrin and cathepsin g that activates map kinase pathway korean actor lee jong sWebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … korean activitiesWebTHP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein … korean actor byeon woo